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Sagewash Effective against Corona Virus & Canine Respiratory Viruses

University of Tennessee College of Veterinary Medicine

Virus

Titer of

Stock

(TCID50)

Titer Post PBS

Treatment

(TCID50) (1:200 dilution of original stock + dialysis; 4 replicates)

Titer Post

Sagewash

Treatment (1:200 dilution of original stock + dialysis; 4 replicates)

Titer Post Clorox

Treatment (1:200 dilution of original stock + dialysis; 4 replicates)

Canine Parainfluenza Virus

(Paramyxoviridae)

107.5/mL

104.5/mL

No growth in

undiluted sample

No growth in

undiluted sample

Canine Distemper Virus

(Paramyxoviridae)

106.7/mL

101.7/mL

No growth in

undiluted sample

No growth in

undiluted sample

Canine Herpesvirus

106.2/mL

103.2/mL

No growth in

undiluted sample

No growth in

undiluted sample

Canine Adenovirus

108.2/mL

106.8/mL

No growth in

undiluted sample

No growth in

undiluted sample

Influenza Virus (H1N1)

(Orthomyxoviridae)

106.5/mL

101.5/mL

No growth in

undiluted sample

No growth in

undiluted sample

Bovine Coronavirus

(Coronaviridae group II, same group as Canine Respiratory Coronavirus)

106.5/mL

103.7/mL

No growth in

undiluted sample

No growth in

undiluted sample

 

Sagewash was evaluated for the inactivation of the canine respiratory viruses with the same protocol described for the previous study. Inactivation ranged from 6.8-1.5 logs, based on the titers of the control samples (diluted 1:100 with PBS, treated with PBS and dialyzed). 

The enveloped RNA viruses are not very stable in the environment (ie canine distemper virus and influenza virus) and did not survive the PBS control procedure well. Several attempts were made to increase the initial titers of these viruses, but increasing the titers had only minimal effects on the outcome of the study. Despite this difficulty, the Sagewash treatment performed as well as a 3% solution of Clorox for the inactivation of canine respiratory viruses.


Melissa Kennedy, DVM, PhD, DACVM Director, Clinical Virology

Associate Professor

Department of Comparative Medicine

UTCVM

Knoxville, TN 37996-4543


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